Dozulé Laboratory for Equine Diseases
Address : 14430 Dozulé
Phone : + 33 (0) 2 31 79 22 76
Manager : Claire Laugier
The Dozulé laboratory for equine diseases concentrates on controlling health risks related to infectious or parasitic diseases in horses through two key focuses:
- epidemiology and epidemiological surveillance of these diseases;
- improving diagnostic and test tools.
Its 30-strong staff works in three research units: bacteriology & parasitology, epidemiology & pathological anatomy, and virology.
The Dozulé Laboratory for Equine Diseases helps improve horse health.
- it is a member of the national epidemiological surveillance network and coordinator of the observatory for equine diseases,
- it monitors the occurrence and/or spread of major and emerging diseases, and analyses their causes, particularly through autopsies and epidemiological investigations in the field,
- it studies infectious agents and gastro-intestinal parasites in horses,
- it develops diagnostic tools and preventive measures for infectious and parasitic diseases,
- as the National Reference Laboratory (NRL) for contagious equine metritis, dourine, equine viral arteritis, equine infectious anaemia and equine herpesviruses, it provides scientific and technical support for veterinary inspections carried out by the authorities, including first- and second-line sample analyses, training of field laboratory staff and monitoring of their analysis quality.
- it has shared the status of European Union Reference Laboratory (EURL) for equine diseases with the Maisons-Alfort Laboratory for Animal Health since July 2008; five of the eight diseases included in the mandate are covered by the Dozulé laboratory, namely contagious equine metritis, dourine, equine infectious anaemia, equine viral arteritis and equine herpesviruses.
- Contagious equine metritis: development, optimisation and/or validation of diagnostic and molecular epidemiological surveillance tools – Taylorella genus genome sequencing - strain characterisation and typing – monitoring of antimicrobial resistance.
- Equine rhodococcosis: development of vaccines – study of the biodiversity of virulence plasmids – study of the molecular mechanisms involved in the early stages of the infection.
- Research into the therapeutic potential of equine antimicrobial peptides to combat the main pathogenic bacteria for horses and other domestic animal species
- Molecular characterisation and typing of strains of equine viral arteritis and rhinopneumonitis (type 1 equine herpesvirus) found in France. Investigation of their pathogenicity.
- Molecular characterisation of strains of equine infectious anaemia found in France and Europe.
- Improvement of serological and molecular diagnostic methods for equine viral arteritis and equine infectious anaemia.
- Prevalence and pathogenicity of the horse’s main digestive parasites – monitoring of the resistance of nematodes to anthelmintics
- Improvement of serological and molecular diagnostic methods for dourine
Exploitation of the autopsy data bank:
- Investigation into the causes of abortion among mares
- Neonatology: study of infectious and non-infectious causes of mortality
- Adult horses: study of the main causes of mortality and sudden death
- Member of the “abortion” subnetwork of RESPE, the French network for epidemiological surveillance of equine diseases, and supplier of information for the associated database
- Study of the nasal carriage of coagulase-positive staphylococci in horses
- Study of the correlation between the concentration of R. equi in aerosol form and prevalence of the disease on stud farms
The Dozulé laboratory carries out reference activities in the following areas:
National Reference Laboratory for:
- contagious equine metritis and dourine
- equine infectious anaemia
- equine viral arteritis and equine herpesviruses (in partnership with the Maisons-Alfort Laboratory for Animal Health)
European Union Reference Laboratory for:
- contagious equine metritis
- equine infectious anaemia
- equine viral arteritis and equine herpesviruses
Type of analyses:
- First- and second-line diagnostic tests on equine contagious metritis (bacteriology, immunofluorescence and PCR)
- First- and second-line diagnostic tests on dourine (serological tests by complement fixation)
- First- and second-line diagnostic tests on equine viral arteritis and equine infectious anaemia (serological tests, viral isolation and RT-PCR)
- Autopsies for diagnostic, expert appraisal or research purposes
- Aerobic bacteriological, mycological and parasitological analyses from autopsy samples
Inter-laboratory proficiency tests (ILPT)
As part of its reference activities concerning contagious equine metritis:
- ILPT for bacteriology: 57 accredited laboratories;
- ILPT for indirect immunofluorescence: 42 accredited laboratories.
As part of its reference activities concerning equine viral arteritis:
- ILPT for serology (serum neutralisation): eight accredited laboratories;
- ILPT for viral isolation: five accredited laboratories.
As part of its reference activities concerning equine infectious anaemia:
- ILPT for serology (Coggins test): seven accredited laboratories.
- Regional Council of Basse-Normandie
- General (Departmental) Council of Calvados
- European Union
- Hippolia competitiveness cluster
- Hippolia foundation for scientific cooperation
- IFCE, the French horse and riding institute
- University and University Hospital of Caen
- GRECAN, the regional cancer investigation group, clinical research unit, Centre François Baclesse, Caen
- INRA, the French institute for agricultural research
- Frank Duncombe departmental laboratory, Saint-Contest
- the four French national veterinary schools
- AVEF, the French equine veterinary association
- RESPE, the French epidemiological surveillance network for equine diseases
- National vocational training centre for stud farms - La jumenterie du Pin, 61310 Exmes
- University of Florida, USA (S. Giguere)
- Gluck Equine Research Center, University of Kentucky, USA (P. Timoney and U. Balasuriya)
- Irish Equine Centre, Johnstown, Ireland (A. Cullinane)
- University of Kiel, Germany
- Institute for tropical medicine, Antwerp, Belgium